This case study explores the potential of nocturnal heritage as a driver for urban regeneration in Valparaíso’s historic port district. Although designated as a UNESCO World Heritage Site in 2003, the area has faced physical and social decline, losing residents and commercial activity. Conducted in 2019 by the Municipality of Valparaíso in collaboration with the Inter-American Development Bank (IDB), the study proposes a revitalisation strategy centred on reactivating symbolic nighttime spaces such as historic bars and the local market. It argues that the night holds cultural, economic, and social value, and that a new governance approach is needed to harness these assets and bring life back to the historic quarter after dark.